Anti-HAS1 Rabbit Polyclonal Antibody for WB, ELISA - Q92839

Core Product Specifications and Parameters

Biological Background: Hyaluronan Synthase 1 (HAS1) Function and Localization

• Hyaluronan synthase 1 (HAS1), encoded by the HAS1 gene, is a glycosyltransferase that catalyzes the synthesis of hyaluronan (hyaluronic acid), a major component of the extracellular matrix.
• HAS1 adds alternating GlcNAc and GlcUA monosaccharides to the growing hyaluronan polymer, playing a critical role in tissue architecture, cell adhesion, migration, and differentiation.
• It is one of three hyaluronan synthase isozymes (HAS1, HAS2, HAS3) in mammals, each with distinct kinetic properties and regulation.
• HAS1 is a multi-pass transmembrane enzyme located in the plasma membrane.
• The protein is widely expressed, with highest levels in ovary, spleen, thymus, prostate, testes, and large intestine; weaker expression in small intestine.
• In addition to hyaluronan synthesis, HAS1 can catalyze the synthesis of chito-oligosaccharides under certain substrate conditions (By similarity).
• The predicted molecular weight is approximately 64.7 kDa (UniProt), often detected at ~65 kDa in western blotting.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 80–390 of human HAS1, a large portion of the protein that likely promotes recognition of the native enzyme. Consider epitope mapping if specific domain binding is required.
• For western blotting, expect a primary band near 65 kDa. Due to possible modifications, validate band identity with appropriate controls (e.g., knockdown or overexpression lysates).
• In ELISA, this polyclonal antibody may serve as a capture or detection reagent. Optimize coating and blocking conditions for your specific assay format.
• Reported cross-reactivity with human and mouse HAS1. For other species, verify sequence homology and test experimentally.
• As with polyclonal antibodies, lot-to-lot variability can occur; validate each new lot in your assay system before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive bridge to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or support for rare target raw-material sourcing, CamelBio streamlines your procurement. Our expertise extends to targets like hyaluronan synthase 1 (HAS1), supporting research in extracellular matrix biology, cell adhesion, and related diagnostic development.