Anti-Haptoglobin (HP) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P00738

Core Product Specifications and Parameters

Biological Background: Haptoglobin Function and Localization

• Haptoglobin is an acute phase protein that captures free hemoglobin released during hemolysis, forming hemoglobin-haptoglobin complexes that are rapidly cleared by macrophages via the CD163 scavenger receptor, facilitating heme iron recycling and preventing kidney damage.
• It also acts as an antioxidant, protecting tissues from oxidative damage induced by free hemoglobin.
• Haptoglobin exhibits antibacterial activity and modulates many aspects of the acute phase response.
• The uncleaved form of the alpha-2 allele, known as zonulin, plays a role in intestinal permeability by regulating tight junction disassembly, influencing the balance between tolerance and immunity to non-self antigens.
• The protein is primarily expressed by the liver and secreted into plasma.
• It is a glycoprotein with Serine protease homolog domains (lacking catalytic activity) and Sushi repeats; key post-translational modifications include disulfide bond formation and glycosylation.
• Multiple isoforms arise from alternative splicing and the common HP1/HP2 genetic polymorphism, leading to structural and functional diversity.
• Mature haptoglobin forms disulfide-linked polymers, and the precursor has a molecular weight of approximately 45 kDa, though observed bands may vary due to processing and polymerization.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is 45 kDa; however, consider that glycosylation and polymer formation may result in higher molecular weight bands or a smear pattern.
• Validate the antibody in appropriate positive control samples, such as human liver tissue lysates or plasma, where haptoglobin is abundantly expressed.
• For immunohistochemistry (IHC-P), standard antigen retrieval methods (e.g., citrate buffer, heat-induced epitope retrieval) may be required for optimal detection.
• Given the reported cross-reactivity with mouse and rat, this antibody can be employed for translational studies; still, it is advisable to confirm reactivity in your specific model system.
• In ELISA applications, pairing with a suitable capture antibody may be necessary; the immunogen region (N-terminal 1–100 amino acids) could guide selection to avoid epitope overlap.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials, including validated antibodies and assay reagents. For projects targeting haptoglobin—an acute phase marker with roles in hemolysis, inflammation, and intestinal permeability—we can source optimized polyclonal antibodies like this rabbit anti-HP antibody, matched antibody pairs, and bulk ancillary reagents. Whether you need rare target antibodies or complete raw material packages for clinical assay development, CamelBio streamlines procurement from concept to clinic.