Anti-HAPLN1 Polyclonal Antibody for WB, IHC-P, ELISA - P10915

Core Product Specifications and Parameters

Biological Background: HAPLN1 Function and Localization

• HAPLN1 (Hyaluronan and proteoglycan link protein 1), also known as cartilage-linking protein 1 (CRTL1) or proteoglycan link protein, is a key structural component of the extracellular matrix.
• It stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix, contributing to cartilage integrity and load-bearing properties.
• The protein contains an immunoglobulin-like domain and a proteoglycan tandem repeat, facilitating binding to hyaluronic acid and proteoglycans.
• HAPLN1 is secreted and localized to the extracellular space and extracellular matrix, where it interacts with components like aggrecan and link protein.
• It is widely expressed in tissues, with particularly high levels in cartilage, and weakly expressed in the brain, according to tissue specificity data.
• Post-translational modifications include disulfide bonds and glycosylation, which may influence its stability and functional interactions.
• Keywords: Disulfide bond, Extracellular matrix, Glycoprotein, Hyaluronic acid, Immunoglobulin domain, Repeat, Secreted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 165-354 of human HAPLN1, a region that may target the link domain; confirm cross-reactivity in your species of interest.
• For Western blotting, the predicted molecular weight is ~40 kDa; validate band specificity using appropriate controls.
• In IHC-P, standard antigen retrieval methods and tissue-specific expression (e.g., cartilage) should be considered; optimize detection conditions.
• ELISA performance depends on coating conditions and sample matrix; test the antibody in your relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and IVD developers with streamlined access to high-quality raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, bulk reagents, and rare-target sourcing. For extracellular matrix and cartilage biology research, our supply network supports projects requiring anti-HAPLN1 antibodies and ancillary reagents. From concept to clinic, we offer procurement and technical consulting to accelerate your assay development.