Anti-HAPLN1 Monoclonal Antibody for WB and ELISA - P10915

Core Product Specifications and Parameters

Biological Background: HAPLN1 Function and Localization

• HAPLN1 (hyaluronan and proteoglycan link protein 1), also known as cartilage-linking protein 1 or proteoglycan link protein, stabilizes aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
• It is a member of the hyaluronan and proteoglycan link protein family, which contains an immunoglobulin-like domain and a hyaluronic acid-binding region.
• HAPLN1 is secreted into the extracellular space and localized within the extracellular matrix, particularly in cartilage.
• The protein is widely expressed in various tissues, but weakly expressed in the brain, consistent with its role in cartilage and other connective tissues.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation, as indicated by its glycoprotein status.
• Structurally, it consists of an immunoglobulin-like domain and tandem repeats that mediate interactions with hyaluronic acid and proteoglycans, facilitating the formation of stable supramolecular complexes in the ECM.

Experimental Guidance and Technical Tips

• The immunogen used to raise this antibody is a synthetic peptide spanning amino acids 250-354 of human HAPLN1, which targets an internal region. Consider this when designing experimental protocols, as the antibody may recognize both native and denatured protein.
• For Western blot (WB) analysis, HAPLN1 has a predicted molecular weight of approximately 40 kDa. We recommend using reducing conditions and validating with appropriate positive controls, such as cartilage tissue lysates or chondrocyte cell lines.
• The antibody is also suitable for ELISA. For sandwich ELISA development, pairing with another antibody targeting a non-overlapping epitope could improve sensitivity.
• Given HAPLN1's role in extracellular matrix stabilization, consider evaluating expression in samples from cartilage, connective tissues, or disease models involving osteoarthritis or rheumatoid arthritis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers, labs, and research institutes with seamless access to high-quality antibodies, antigens, and ancillary reagents. For your research on hyaluronan biology and extracellular matrix disorders, we supply validated monoclonal antibodies like this HAPLN1 antibody, as well as related cartilage matrix proteins, to accelerate your assay development. Our extensive portfolio and sourcing capabilities ensure you get the right materials, from concept to clinic.