Anti-HAO1/GOX Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9UJM8

Core Product Specifications and Parameters

Biological Background: HAO1/GOX Function and Localization

• HAO1/GOX, also known as glycolate oxidase (GOX) or glyoxylate oxidase, is a peroxisomal enzyme that belongs to the (S)-2-hydroxy-acid oxidase family.
• It exhibits broad substrate specificity, preferentially oxidizing glycolate with high catalytic efficiency. Related references: PMID:10777549 PMID:10978532 PMID:17669354
• The oxidation of glycolate produces glyoxylate, which is then utilized by alanine-glyoxylate aminotransferase for peroxisomal glycine synthesis, serving as a critical step in glyoxylate detoxification to prevent oxalate accumulation and kidney stone formation. Related references: PMID:10978532 PMID:17669354
• In addition to glycolate, it can also oxidize glyoxylate and long-chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, though with lower catalytic efficiency. Related references: PMID:10777549 PMID:17669354 PMID:18215067
• The enzyme uses molecular oxygen (O2) as the physiological electron acceptor, generating hydrogen peroxide (H2O2) in the process, but can also transfer electrons to artificial acceptors like 2,6-dichlorophenolindophenol (DCIP) in vitro. Related references: PMID:10777549 PMID:10978532 PMID:17669354
• HAO1/GOX shows no activity against L-lactate or 2-hydroxybutanoate. Related references: PMID:10777549
• It is localized to the peroxisome matrix and is highly expressed in the liver.
• The protein is a flavoprotein that binds FMN and is known to be phosphorylated, as identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-370 of human HAO1/GOX, which represents nearly the full-length protein and is expected to generate antibodies recognizing multiple epitopes.
• For Western blot (WB), the predicted molecular weight is 41 kDa, but post-translational modifications or isoforms may cause slight shifts; validate using positive controls such as human liver lysates.
• In immunofluorescence (IF) and immunohistochemistry (IHC), consider co-staining with peroxisomal markers (e.g., PMP70) to confirm peroxisomal localization, and use liver tissue sections known to express high levels of HAO1.
• The antibody has reported cross-reactivity with mouse and rat, making it suitable for studies in these model organisms; validate reactivity in your specific sample system before large-scale experiments.
• As a polyclonal antibody, performance may vary between lots; consider including appropriate controls and optimizing dilutions for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers, laboratories, and research institutes, providing one-stop access to high-quality IVD raw materials. This anti-HAO1/GOX polyclonal antibody is part of our extensive portfolio optimized for metabolic research and kidney stone-related assays. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and offer custom sourcing for rare targets—helping you bridge the gap from concept to clinic with reliable material support.