Anti-HAO1/GOX Monoclonal Antibody for WB, IHC-P, ELISA - Q9UJM8

Core Product Specifications and Parameters

Biological Background: HAO1/GOX Function and Localization

• HAO1 encodes 2-hydroxyacid oxidase 1, a peroxisomal flavoprotein also known as glycolate oxidase (GOX) and glyoxylate oxidase.
• It catalyzes the oxidation of glycolate to glyoxylate using O2 as electron acceptor, producing H2O2; this reaction is central to hepatic glyoxylate detoxification and glycine synthesis. Related references: PMID:10777549 PMID:10978532 PMID:17669354
• The enzyme exhibits broad substrate specificity, also oxidizing glyoxylate and long-chain 2-hydroxyacids (e.g., 2-hydroxyhexadecanoate, 2-hydroxyoctanoate) with lower efficiency. Related references: PMID:10777549 PMID:17669354 PMID:18215067
• It does not utilize L-lactate or 2-hydroxybutanoate as substrates. Related references: PMID:10777549
• HAO1 is localized to the peroxisome matrix, consistent with its role in glyoxylate metabolism.
• The protein is highly expressed in the liver, where glyoxylate detoxification primarily occurs.
• Post-translational modifications include phosphorylation, and it contains an FMN cofactor, classifying it as a flavoprotein oxidoreductase.
• Its predicted molecular weight is ~41 kDa, matching the observed band in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human HAO1; therefore, the antibody is expected to detect full-length HAO1 in denaturing Western blots as a ~41 kDa band.
• For IHC-P, antigen retrieval may be required; test with appropriate positive controls (e.g., human liver tissue) where HAO1 is highly expressed.
• In ELISA, the antibody can be used for detection of recombinant or native HAO1; consider pairing with a capture antibody in a sandwich format.
• Validate cross-reactivity in mouse samples, as indicated; ensure optimal dilutions and conditions are determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with reliable access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-HAO1/GOX antibody. Whether you need optimized antibody pairs for immunoassays, bulk ancillary reagents, or rare target raw-material sourcing, CamelBio's comprehensive supply chain and technical support help accelerate your assay development from concept to clinic. Explore our full portfolio of peroxisomal and metabolic enzyme antibodies for your next diagnostic project.