Anti-HAND2 Polyclonal Antibody for WB, IF, IHC, ELISA - P61296

Core Product Specifications and Parameters

Biological Background: HAND2 Function and Localization

• Protein identity and isoforms: Heart- and neural crest derivatives-expressed protein 2 (HAND2), also known as Class A basic helix-loop-helix protein 26 (bHLHa26) or dHAND, is encoded by the HAND2 gene. Alternative splicing produces multiple isoforms.
• Core transcriptional function: HAND2 is a transcription factor that binds DNA on E-box consensus sequence 5'-CANNTG-3', regulating target gene expression crucial for development.
• Cardiac and vascular development: Essential for cardiac morphogenesis, specifically the formation of the right ventricle and aortic arch arteries. It is required for vascular development and angiogenesis, possibly through a VEGF signaling pathway.
• Limb and branchial arch development: Plays a key role in limb development by establishing anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Also involved in branchial arch development, which gives rise to head and neck structures.
• Subcellular localization: Predominantly localizes to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Tissue expression and functional keywords: Expressed in the heart. Functional keywords include developmental protein, differentiation, angiogenesis, transcription regulation, and DNA-binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 151-217 of human HAND2, targeting a region within the C-terminal portion of the protein. This region includes the basic helix-loop-helix domain, suggesting the antibody may recognize the active DNA-binding form.
• For Western blot (WB), the predicted molecular weight of HAND2 is approximately 24 kDa, but actual migration may vary due to post-translational modifications or isoform differences. Include appropriate positive controls (e.g., heart tissue lysates from human, mouse, or rat) and validate in your sample system.
• Immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P) should reveal nuclear staining. Optimize fixation and permeabilization conditions for your cells/tissues, and consider antigen retrieval for IHC-P.
• This polyclonal antibody has been reported for use in ELISA; users should determine optimal coating concentrations and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering one-stop access to validated antibodies, antigen pairs, and ancillary reagents for diagnostic development. For HAND2, a critical transcription factor in cardiac and limb morphogenesis, we can supply optimized polyclonal antibodies like this HAND2 Rabbit pAb, along with monoclonal alternatives and custom solutions to support your research and assay needs. Whether you require bulk quantities or rare-target raw materials, our sourcing network bridges the gap from concept to clinic.