Anti-HAGH Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q16775

Core Product Specifications and Parameters

Biological Background: HAGH Function and Localization

• HAGH encodes hydroxyacylglutathione hydrolase, a mitochondrial and cytoplasmic enzyme also known as glyoxalase II (Glx II).
• The enzyme functions as a thiolesterase, catalyzing the hydrolysis of S-D-lactoyl-glutathione into glutathione and D-lactic acid, a critical step in the glyoxalase pathway for detoxifying methylglyoxal.
• HAGH belongs to the metallo-beta-lactamase superfamily and requires zinc ions for its catalytic activity, with active site zinc binding essential for function.
• Subcellular localization includes the mitochondrial matrix and cytoplasm, consistent with its role in both compartments to metabolize toxic alpha-oxoaldehydes.
• Alternative splicing and alternative initiation generate multiple isoforms, contributing to functional diversity and tissue-specific regulation.
• Post-translational modifications include acetylation, and the protein has been identified through direct protein sequencing and proteomics approaches.
• Tissue expression is prominent in liver and kidney, organs with high detoxification activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–260 of human HAGH, encompassing a large portion of the protein, which may help generate antibodies recognizing multiple epitopes.
• For western blot, a band around 34 kDa is expected; consider optimizing lysate preparation to preserve mitochondrial fractions if targeting the mitochondrial pool.
• For immunofluorescence/immunocytochemistry, include appropriate controls to confirm either mitochondrial or cytoplasmic staining patterns depending on cell type and fixation conditions.
• ELISA performance should be validated with recombinant protein or known positive controls, as polyclonal antibodies can exhibit lot-to-lot variability; cross-reactivity with mouse and rat homologs may be exploited for multispecies assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to a comprehensive portfolio of IVD raw materials. For targets like HAGH, involved in detoxification and metabolic disease research, we supply validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Whether you require ready-to-use antibodies or rare-target raw-material sourcing, our team is equipped to bridge your project from concept to clinic.