Anti-HADHB Monoclonal Antibody (Rabbit) for WB, IHC-P, IF/ICC, ELISA - P55084

Core Product Specifications and Parameters

Biological Background: Trifunctional enzyme subunit beta, mitochondrial Function and Localization

• HADHB (TP-beta) encodes the beta subunit of the mitochondrial trifunctional enzyme, a heterotetrameric complex that catalyzes the final three steps of long-chain fatty acid β-oxidation. The alpha subunit (HADHA) contributes enoyl-CoA hydratase and hydroxyacyl-CoA dehydrogenase activities, while HADHB carries out 3-ketoacyl-CoA thiolysis. Related references: PMID:16423905, PMID:26474213, PMID:29915090
• Each β-oxidation cycle removes two carbons from the fatty acyl-CoA chain, producing acetyl-CoA, FADH₂, and NADH. These electron carriers drive ATP synthesis via the citric acid cycle and oxidative phosphorylation, meeting high energy demands in tissues such as heart and muscle. Related references: PMID:26474213, PMID:29915090
• The trifunctional enzyme preferentially acts on long-chain substrates, including saturated fatty acids like palmitate and laurate. Experimental evidence confirms thiolase activity against select substrates, and its catalytic mechanism suggests activity on unsaturated fatty acids such as oleate, linoleate, and linolenate. Related references: PMID:1550553, PMID:8135828, PMID:8163672, PMID:30850536
• HADHB localizes to the mitochondrial inner membrane, outer membrane, and endoplasmic reticulum, indicating a dynamic role in lipid metabolism across organelle contact sites.
• Post-translational modifications include acetylation, and alternative splicing generates multiple isoforms. Mutations in HADHB are linked to mitochondrial trifunctional protein deficiency, a severe metabolic disorder.
• The protein has a calculated molecular weight of approximately 51.3 kDa (observed ~51 kDa) and is conserved across human, mouse, and rat.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 331–474 of human HADHB, a region conserved in mouse and rat; this likely accounts for the reported cross-species reactivity. For species-specific experiments, confirm sequence alignment and validate detection in relevant tissue lysates.
• For Western blot, consider using mitochondrial-enriched fractions from heart or liver tissue and a recommended primary antibody dilution between 1:1000 and 1:5000, optimizing for your sample type.
• In IHC-P, antigen retrieval (e.g., citrate buffer, pH 6.0) may be required. Include a known positive control tissue such as cardiac muscle.
• For IF/ICC, co-staining with a mitochondrial marker (e.g., TOM20) is advisable to verify localization. Permeabilization with Triton X-100 or digitonin may be needed.
• When used in ELISA, pair with a matched detection antibody for sandwich assays. Assess cross-reactivity with other thiolase family members if relevant.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this validated rabbit monoclonal anti-HADHB antibody for research into mitochondrial fatty acid β-oxidation and related metabolic disorders. As a comprehensive IVD raw-material provider, we offer a full range of optimized antibodies, bulk ancillary reagents, and custom sourcing for challenging targets, helping you bridge discovery to diagnostic development efficiently.