Anti-HADHA Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P40939

Core Product Specifications and Parameters

Biological Background: HADHA Function and Localization

• HADHA encodes the alpha subunit of the mitochondrial trifunctional enzyme, which catalyzes the last three steps of mitochondrial beta-oxidation of long-chain fatty acids, providing acetyl-CoA, NADH, and FADH(2) for energy production. Related references: PMID:1550553, PMID:29915090, PMID:30850536
• The protein forms a heterotetramer with HADHB; HADHA carries enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase activities, while HADHB possesses 3-ketoacyl-CoA thiolase activity. Related references: PMID:29915090, PMID:30850536, PMID:8135828
• It is specific for long-chain saturated fatty acids (e.g., palmitate, laurate) and also predicted to act on long-chain unsaturated fatty acids (e.g., oleate, linoleate). Related references: PMID:1550553, PMID:8135828, PMID:8163672, PMID:26474213
• HADHA also exhibits a cardiolipin acyltransferase activity, participating in mitochondrial cardiolipin remodeling, potentially downstream of tafazzin. Related references: PMID:23152787, PMID:31604922
• The protein localizes to the mitochondrial inner membrane and contains a transit peptide for mitochondrial import.
• Keywords: Multifunctional enzyme, fatty acid metabolism, lipid metabolism, lyase, oxidoreductase, transferase; post-translational modifications include acetylation, methylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 545–763 of human HADHA, a region within the 3-hydroxyacyl-CoA dehydrogenase domain; thus, the antibody may recognize both full-length HADHA and C-terminal fragments.
• For Western blotting, the predicted molecular weight is 83 kDa; validate antibody specificity in human or mouse lysates with appropriate controls.
• For IHC-P and IF/ICC, consider testing various fixation and antigen retrieval conditions to optimize mitochondrial staining.
• ELISA reactivity against the recombinant immunogen suggests suitability for quantitative detection; consider pairing with a capture antibody for sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your IVD development with one-stop access to high-quality raw materials, including validated HADHA antibodies for mitochondrial fatty acid oxidation studies. From optimized polyclonal and monoclonal antibodies to bulk ancillary reagents and rare target sourcing, we help streamline your workflow from research to diagnostic application.