Anti-HADHA Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P40939

Core Product Specifications and Parameters

Biological Background: HADHA Function and Localization

• HADHA encodes the alpha subunit of the mitochondrial trifunctional enzyme, catalyzing the hydration and dehydrogenation steps of long-chain fatty acid beta-oxidation. Related references: PMID:1550553, PMID:29915090, PMID:30850536
• The enzyme complex (with HADHB) processes long-chain saturated fatty acids (e.g., palmitate and laurate) and is predicted to act on unsaturated fatty acids like oleate and linoleate. Related references: PMID:30850536, PMID:31604922, PMID:1550553, PMID:8135828, PMID:8163672, PMID:26474213
• Each beta-oxidation cycle shortens fatty acyl-CoA by two carbons, yielding acetyl-CoA, FADH₂, and NADH, linking to the citric acid cycle and electron transport chain. Related references: PMID:29915090
• HADHA also acts as a monolysocardiolipin acyltransferase (MLCL AT), remodeling cardiolipin in the mitochondrial inner membrane, potentially with Tafazzin. Related references: PMID:23152787, PMID:31604922
• The protein localizes to the mitochondrial inner membrane and undergoes post-translational modifications including acetylation, phosphorylation, and methylation.
• Alternative splicing generates multiple isoforms, and UniProt annotates HADHA as a disease variant gene, indicating potential involvement in metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 484-763 of human HADHA; this C-terminal region may contain key functional domains, enhancing detection specificity.
• For Western blotting, the predicted molecular weight is approximately 83 kDa; verify protein expression in appropriate tissue lysates (e.g., liver, heart, muscle) rich in mitochondria.
• In immunofluorescence (IF/ICC), expect mitochondrial staining; use MitoTracker co-staining to confirm localization.
• Since the antibody is polyclonal, lot-to-lot variation may occur; consider initial titration experiments for each new application.
• ELISA and IP workflows have been reported; validate antibody binding under your experimental conditions using known positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive selection of IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. Whether you need ready-to-use anti-HADHA antibodies for mitochondrial metabolism research or seek customized bulk sourcing of polyclonal antibodies, CamelBio streamlines your procurement from concept to clinic. Our expert team supports your projects with technical consultation and reliable supply chain management.