Anti-HADH Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q16836

Core Product Specifications and Parameters

Biological Background: HADH Function and Localization

• HADH (Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial) catalyzes the third step of the mitochondrial beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10), essential for energy production from fatty acids (PubMed:10231530, PubMed:11489939, PubMed:16725361). Related references: PMID:10231530 PMID:11489939 PMID:16725361
• Also designated as medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase or short-chain 3-hydroxyacyl-CoA dehydrogenase (SCHAD), the protein plays a central role in lipid metabolism.
• Beyond beta-oxidation, HADH negatively regulates insulin secretion by inhibiting glutamate dehydrogenase 1 (GLUD1) activation, linking fatty acid metabolism to glucose homeostasis (By similarity).
• The enzyme contributes to the maintenance of normal spermatogenesis by reducing fatty acid accumulation in the testes, highlighting a specialized role in reproductive tissues (By similarity).
• HADH localizes to the mitochondrial matrix, where it functions as an NAD-dependent oxidoreductase.
• Post-translational modifications include acetylation and hydroxylation, which may modulate enzymatic activity or stability.
• Tissue expression is highest in liver, kidney, pancreas, heart, and skeletal muscle, consistent with tissues having high energy demands or specialized metabolic functions.
• Mutations in HADH are associated with disease variants such as hyperinsulinemic hypoglycemia, emphasizing its clinical relevance in metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen encompasses full-length human HADH (aa 1–314); while it may cross-react with mouse and rat orthologs, species-specific validation is recommended.
• For western blotting, anticipate a specific band near 34 kDa; optimize gel percentage and transfer conditions for low-molecular-weight proteins.
• In IHC-paraffin applications, consider testing different antigen retrieval methods and antibody dilutions on positive control tissues such as human liver or kidney.
• For ELISA, titrate the antibody against recombinant HADH or native protein in the relevant sample matrix to establish optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic one-stop partner for diagnostic manufacturers and research institutes, providing validated IVD raw materials like this anti-HADH polyclonal antibody for metabolic disease and mitochondrial research. Our comprehensive catalogue includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing support to accelerate your assay development pipeline.