Anti-HACE1 Polyclonal Antibody for WB, IHC-P, ELISA - Q8IYU2

Core Product Specifications and Parameters

Biological Background: HACE1 Function and Localization

• HACE1 is an E3 ubiquitin-protein ligase that regulates Golgi membrane fusion and small GTPase signaling (PubMed:15254018, PubMed:21988917, PubMed:22036506).
  Related references:
  PMID:15254018
  PMID:21988917
  PMID:22036506

• It acts as a key regulator of Golgi membrane dynamics during the cell cycle; it is recruited to the Golgi by Rab proteins and mediates ubiquitination during mitotic Golgi disassembly, providing a signal for Golgi reassembly after cell division (PubMed:21988917).
  Related references:
  PMID:21988917

• HACE1 specifically binds GTP-bound RAC1 and promotes its ubiquitination and degradation, thereby contributing to host defense against pathogens (PubMed:22036506, PubMed:37537642, PubMed:38332367).
  Related references:
  PMID:22036506
  PMID:37537642
  PMID:38332367

• It may also function as a transcription regulator through interaction with RARB (By similarity).

• Subcellularly, HACE1 localizes to the Golgi apparatus, Golgi stack membrane, cytoplasm, and endoplasmic reticulum.

• The protein contains ankyrin (ANK) repeats and a HECT domain, characteristic of E3 ubiquitin ligases; alternative splicing yields multiple isoforms.

• HACE1 is expressed in multiple tissues including heart, brain, and kidney.

• Mutations in HACE1 are linked to hereditary spastic paraplegia and chromosomal rearrangements observed in several cancers, highlighting its role in neurodegeneration and tumor suppression.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the N-terminal region (amino acids 1–100) of human HACE1; consider verifying sequence conservation if using a non-human sample system.
• For Western blot (WB), the predicted molecular weight of HACE1 is approximately 102 kDa; include appropriate molecular weight markers and validate the band identity using known positive controls.
• When performing immunohistochemistry (IHC-P), note the Golgi and cytoplasmic localization of HACE1; appropriate subcellular staining patterns should be confirmed.
• The reported cross-reactivity with mouse and rat suggests epitope conservation; however, always validate in the relevant species and tissue context.
• For ELISA applications, optimize coating antigen and antibody dilution using standard checkerboard titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material supply, offering tailored sourcing solutions from concept to clinic. We provide validated antibodies, including optimized polyclonal and monoclonal antibodies targeting HACE1 and other ubiquitylation-related proteins, along with bulk ancillary reagents. Whether you need rare-target antibody development or large-scale production, CamelBio simplifies procurement with technical consultation to accelerate your diagnostic and research workflows.