Anti-H3F3B Rabbit Polyclonal Antibody for WB, ELISA - P84243

Core Product Specifications and Parameters

Biological Background: Histone H3.3 Function and Localization

• Histone H3.3 is a variant of histone H3, encoded by two distinct genes: H3-3A (H3F3A) and H3-3B (H3F3B).
• It replaces conventional histone H3 in a wide range of nucleosomes in active genes and is the predominant H3 form in non-dividing cells.
• H3.3 is deposited into chromatin independently of DNA synthesis, marking sites of nucleosomal displacement and transcriptionally active chromatin.
• As a core component of nucleosomes, Histone H3.3 plays a central role in compacting DNA into chromatin, thereby regulating transcription, DNA repair, replication, and chromosomal stability.
• Localized in the nucleus and associated with chromosomes.
• Subject to diverse post-translational modifications including acetylation, methylation, phosphorylation, citrullination, and others, which constitute the histone code and regulate DNA accessibility.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to amino acids 1-136 of human H3F3B. Ensure that the antibody is validated for your specific application and sample preparation method.
• For WB, the predicted protein weight is approximately 15 kDa. Use appropriate gel percentages and loading controls.
• This polyclonal antibody may exhibit cross-reactivity with other histone H3 variants due to high sequence homology; verify specificity using appropriate controls.
• Consider optimizing antibody dilution and blocking conditions for your specific experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, providing validated antibodies, bulk reagents, and rare target procurement. Our H3F3B antibody supports chromatin and epigenetic research applications. We offer comprehensive supply solutions from concept to clinic, ensuring reliable access to the materials you need.