Anti-H2-Ab1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P06345

Core Product Specifications and Parameters

Biological Background: H2-Ab1 Function and Localization

• H2-Ab1 encodes the beta chain of the mouse H-2 class II histocompatibility antigen, A-S, a major histocompatibility complex (MHC) class II molecule.
• MHC class II molecules present extracellular antigenic peptides to CD4+ helper T cells, thereby orchestrating adaptive immune responses.
• The protein is a transmembrane glycoprotein that resides on the cell surface, with a signal peptide directing its co-translational insertion into the endoplasmic reticulum.
• It contains conserved structural features including disulfide bonds and a transmembrane helix, characteristic of the MHC class II family.
• Ubiquitin-like (Ubl) conjugation has been identified, suggesting post-translational regulation of protein stability or trafficking.
• This gene product is part of the Swiss-Prot reference proteome, ensuring high-quality annotation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30-219, which corresponds to the extracellular domain; consider its suitability for detecting surface-expressed or soluble MHC class II complexes.
• For Western blotting, a band around 30 kDa is expected; however, glycosylation may cause electrophoretic mobility shifts. Validate the observed band pattern in your model system.
• In immunohistochemistry or immunofluorescence, antigen retrieval optimization may be required, especially for formalin-fixed paraffin-embedded tissues.
• Cross-reactivity with human and rat is reported; experimental validation in the relevant species and tissue is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic and research communities by providing streamlined access to premium IVD raw materials and tailored technical support. This anti-H2-Ab1 antibody supports investigations into MHC class II biology, transplantation immunology, and autoimmune disease models. Our sourcing capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw material procurement—offering a dependable bridge from concept to clinic.