Anti-GZMA Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P12544

Core Product Specifications and Parameters

Biological Background: Granzyme A Function and Localization

• Granzyme A, also known as CTL tryptase, fragmentin-1, Hanukkah factor (HF), and encoded by the GZMA gene (CTLA3, HFSP), is an abundant serine protease found in the cytoplasmic granules of cytotoxic T-cells and natural killer (NK) cells.
• It induces caspase-independent pyroptosis in target cells after being delivered via the immunological synapse, cleaving after Lys or Arg residues. Related references: PMID:12819770, PMID:32299851, PMID:3257574
• Once inside the target cell, GZMA cleaves gasdermin-B (GSDMB), releasing its pore-forming moiety to trigger pyroptosis and cell death. Related references: PMID:32299851, PMID:34022140, PMID:36157507
• It also cleaves APEX1 after Lys-31, destroying its oxidative repair activity. Related references: PMID:12524539
• GZMA cleaves the nucleosome assembly protein SET after Lys-189, disrupting nucleosome assembly and allowing the SET complex to migrate into the nucleus to nick and degrade DNA. Related references: PMID:11555662, PMID:12628186, PMID:16818237
• Subcellularly, granzyme A is localized to cytoplasmic granules and is secreted upon degranulation.
• As a disulfide-bonded glycoprotein, it is initially produced as a zymogen and is a well-characterized serine protease with available 3D structures.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 29 kDa is expected; validate detection using positive control lysates from activated cytotoxic T cells or NK cell lines.
• In immunofluorescence/immunocytochemistry, permeabilization is required to detect granular cytoplasmic GZMA. Co-staining with perforin or CD8 may confirm cytotoxic cell specificity.
• When performing ELISA, consider using recombinant human GZMA as a standard and verify linearity in the relevant sample matrix.
• This antibody shows cross-reactivity with mouse and rat GZMA, but sequence alignment of the immunogen region is advised if quantitative interspecies comparisons are needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to IVD raw materials, including this anti-GZMA polyclonal antibody for immunology and cell death applications. We offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents to support your assay development from concept to clinic. For customized sourcing of rare targets or technical consultation, contact our supply chain team.