Anti-GZMA Rabbit Monoclonal Antibody for WB, IP, IF/ICC, ELISA - P12544

Core Product Specifications and Parameters

Biological Background: Granzyme A Function and Localization

• Granzyme A (GZMA), also known as CTL tryptase, fragmentin-1, and Hanukkah factor (HF), is a serine protease abundantly expressed in the cytosolic granules of cytotoxic T-cells and NK-cells.
• Upon delivery into target cells through the immunological synapse, GZMA triggers caspase-independent pyroptosis by cleaving gasdermin-B (GSDMB), releasing its pore-forming moiety to induce target cell lysis (PubMed:32299851, PubMed:34022140, PubMed:36157507).
  Related references:
  PMID:32299851 PMID:34022140 PMID:36157507
• It cleaves after Lys or Arg residues (PubMed:12819770, PubMed:32299851).
  Related references:
  PMID:12819770 PMID:32299851
• Additional substrates include the oxidative repair enzyme APEX1, cleaved at Lys-31 (PubMed:12524539), and the nucleosome assembly protein SET, cleaved at Lys-189 to promote DNA degradation (PubMed:11555662, PubMed:12628186, PubMed:16818237).
  Related references:
  APEX1: PMID:12524539
  SET: PMID:11555662 PMID:12628186 PMID:16818237
• GZMA localizes to cytoplasmic granules and is secreted into the immunological synapse upon target cell recognition.
• The protein is a glycoprotein containing disulfide bonds and is initially synthesized as an inactive zymogen that requires proteolytic processing for activation.

Experimental Guidance and Technical Tips

• For western blotting, expect a band at approximately 29 kDa under reducing conditions; validate in relevant human cell lines such as cytotoxic T cells or NK cells.
• When performing immunofluorescence or immunocytochemistry, consider permeabilization to visualize granular cytoplasmic staining.
• For immunoprecipitation and ELISA, optimize antibody dilution in your specific sample matrix.
• Ensure appropriate positive and negative controls (e.g., GZMA-expressing vs. knockout cell lines) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing bridge for IVD raw materials, connecting diagnostic manufacturers and researchers with high-quality reagents like this anti-GZMA monoclonal antibody. Our comprehensive portfolio includes validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing for rare targets. Partner with us to accelerate your immunology and oncology diagnostic development from concept to clinic.