Anti-GYPB Rabbit Polyclonal Antibody for WB, ELISA - P06028

Core Product Specifications and Parameters

Biological Background: Glycophorin-B Function and Localization

• Glycophorin-B (GYPB, also known as SS-active sialoglycoprotein, PAS-3, and CD235b) is a single-pass transmembrane sialoglycoprotein expressed on erythrocytes.
• It is a component of the ankyrin-1 complex, a multiprotein complex critical for maintaining the stability and shape of the erythrocyte membrane.
• The GYPB gene belongs to the glycophorin gene family (including GYPA and GYPE) and encodes the S and s blood group antigens of the MNS system.
• GYPB is localized exclusively to the cell membrane of erythrocytes.
• The mature protein undergoes extensive O-linked sialylation, contributing to its negative charge and role in red blood cell membrane organization.
• Alternative splicing generates multiple isoforms; the reviewed UniProt canonical isoform has a calculated molecular weight of ~9.8 kDa.
• UniProt keywords highlight 3D-structure determination, blood group antigen activity, sialic acid modification, and a transmembrane helix topology.

Experimental Guidance and Technical Tips

• For western blotting, consider using a 15% SDS-PAGE gel and low molecular weight markers to resolve the ~10–15 kDa glycoprotein band; the heavily glycosylated native form may migrate somewhat higher than the predicted core protein weight.
• When performing ELISA, the synthetic peptide immunogen (N-terminal region) may be used as a positive control; recombinant GYPB extracellular domain can also serve as a target.
• Because the antibody is raised against an N-terminal peptide and cross-reacts with mouse GYPB, it may detect multiple isoforms or processing variants; validate specificity in the relevant sample system using appropriate controls.
• For membrane protein detection, consider preparing erythrocyte ghost membranes or enriching membrane fractions to improve signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-GYPB polyclonal antibody. Supporting erythrocyte membrane research and MNS blood group antigen studies, we provide ready-to-use antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare-target raw materials. Let CamelBio streamline your supply chain from concept to clinic.