Anti-GYPB Polyclonal Antibody for IF/ICC, ELISA - P06028

Core Product Specifications and Parameters

Biological Background: GYPB Function and Localization

• Glycophorin-B (GYPB), also known as SS-active sialoglycoprotein or CD235b, is a single-pass transmembrane sialoglycoprotein predominantly expressed on the surface of red blood cells.
• It is a key component of the ankyrin-1 complex, a multiprotein assembly critical for maintaining the stability and shape of the erythrocyte membrane.
• GYPB carries several blood group antigens, including the S and s antigens (MNS blood group system), which arise from genetic polymorphisms.
• The protein is heavily glycosylated with sialic acid residues, contributing to its negative charge and role in preventing erythrocyte aggregation.
• Alternative splicing of the GYPB gene generates multiple transcript variants, potentially giving rise to protein isoforms with different functional properties.
• Subcellularly, GYPB is anchored in the cell membrane via its transmembrane helix, with its N-terminal glycodomain exposed extracellularly and a short C-terminal tail in the cytoplasm.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 1–91). For IF/ICC, consider using non-permeabilizing conditions to detect surface-localized GYPB.
• Validate antibody specificity in relevant human erythrocyte samples or GYPB-expressing cell lines to confirm reactivity.
• In ELISA applications, recombinant GYPB protein or erythrocyte membrane extracts may serve as suitable coating antigens; optimize coating conditions and antibody dilutions for your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-GYPB reagent, and bulk ancillary supplies. Leveraging deep erythrocyte biology expertise, we streamline procurement for blood group antigen and membrane stability studies. Whether you need rare target raw-material sourcing or one-stop technical support, CamelBio bridges your project from concept to clinic.