Anti-GYG1 Rabbit Polyclonal Antibody for WB and ELISA - P46976

Core Product Specifications and Parameters

Biological Background: Glycogenin-1 Function and Localization

• Glycogenin-1 (GYG1), also known as GYG or GSD15, is a key enzyme in glycogen biosynthesis, catalyzing the formation of a short α-1,4-glucosyl primer on a tyrosine residue for glycogen synthase.
• It exists in multiple isoforms due to alternative splicing, potentially contributing to functional diversity in glycogen metabolism.
• GYG1 localizes to both the cytoplasm and nucleus, enabling glycogen priming in different subcellular compartments.
• Post-translational modifications, including acetylation and phosphorylation, may regulate its enzymatic activity and protein interactions.
• Highly expressed in skeletal muscle and heart, with lower levels detected in brain, lung, kidney, and pancreas.
• Mutations in GYG1 are associated with glycogen storage disease, underscoring its critical role in glycogen homeostasis.
• The protein binds manganese as a cofactor and functions as a glycoprotein and transferase.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 234–333 of human GYG1; this region may be conserved in mouse and rat, consistent with the observed cross-reactivity.
• For Western blot, a band around 39 kDa is expected; consider using lysates from skeletal muscle or heart where GYG1 is highly expressed.
• In ELISA applications, titration and buffer optimization are recommended for each sample type.
• As a polyclonal antibody, it may offer robust detection but could exhibit lot-to-lot variation; appropriate controls are advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-GYG1 polyclonal antibody supports research in glycogen metabolism and glycogen storage disorders. Our one-stop sourcing model bridges your project from concept to clinic with reliable supply and technical insight.