Anti-GUK1 Rabbit Polyclonal Antibody for WB, ELISA - Q16774

Core Product Specifications and Parameters

Biological Background: GUK1 Function and Localization

• GUK1 catalyzes the phosphorylation of GMP to GDP, a critical step for recycling GMP and indirectly cGMP, and has been implicated in the survival and growth progression of some tumors. Related references: PMID:31201273

• In photoreceptors, GUK1 contributes to cGMP metabolism, supporting visual signal transduction (by similarity).

• GUK1 is essential for converting antiviral and anticancer prodrugs such as acyclovir, ganciclovir, 6-thioguanine, and 6-mercaptopurine into their pharmacologically active metabolites. Related references: PMID:197968, PMID:6248551, PMID:6306664

• The protein localizes to the photoreceptor inner segment, cytosol, cytoplasm, and mitochondria.

• Widely expressed across tissues.

• GUK1 is an ATP- and nucleotide-binding kinase with transferase activity; alternative splicing produces multiple isoforms, and the protein undergoes acetylation.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1–197) of human GUK1, which contains the core catalytic domain; verify detection of the full-length protein at the predicted molecular weight of ~22 kDa in Western blot under reducing conditions.
• For Western blot, use appropriate human cell or tissue lysates as the primary test system; perform a no-primary control to confirm band specificity.
• For ELISA, titrate the antibody against the recombinant immunogen or native GUK1 to establish an optimal coating concentration and detection range; consider pairing with a matched detection antibody for sandwich ELISA development.
• Cross-reactivity is reported for human; when applying to other species, validate reactivity experimentally as sequence homology may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, technical services, and procurement support. For targets like GUK1 involved in nucleotide metabolism and prodrug activation, CamelBio can source validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to accelerate assay development. Contact us to discuss custom sourcing for rare or specialized targets.