Anti-GUCY2F Rabbit Polyclonal Antibody for WB and ELISA - P51841

Core Product Specifications and Parameters

Biological Background: GUCY2F Function and Localization

• Encodes a retinal guanylyl cyclase (ROS-GC2) responsible for synthesizing cyclic GMP (cGMP) in rod and cone photoreceptors, a critical step in phototransduction and cGMP replenishment after light activation (PubMed:7777544). Related references: PMID:7777544
• Plays an essential role in vision by converting GTP to cGMP, which regulates cyclic nucleotide-gated channels in photoreceptor outer segments; also implicated in trafficking of membrane-associated proteins to the outer segment.
• Member of the guanylate cyclase family; alternative names include GUC2F, GC-F, RETGC-2, and ROS-GC2, indicating its specific expression in retinal photoreceptors.
• Localized exclusively to the photoreceptor outer segment membrane, a specialized sensory cilium where phototransduction occurs, as confirmed by tissue-specific expression in the outer nuclear layer and inner segments of rod and cone cells.
• Contains a signal peptide, a single transmembrane helix, a protein kinase-like domain, and a catalytic guanylyl cyclase domain, characteristic of membrane-bound guanylate cyclases; post-translational disulfide bond formation is predicted.
• Keywords highlight its roles in sensory transduction, vision, cGMP biosynthesis, and lyase activity; proteomics identification supports its expression in human retina.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 250–460, which corresponds to the intracellular kinase homology domain; consider using this antibody to detect full-length GUCY2F (~125 kDa) in retinal or photoreceptor-derived cell lysates.
• Cross-reactivity with mouse and rat is reported; validate in appropriate tissue samples if species-specific detection is required.
• For Western blot, standard reducing conditions are recommended, but non-reducing conditions may reveal disulfide-linked multimers; optimize loading controls for high-molecular-weight proteins.
• For ELISA, use as a capture or detection antibody in indirect or sandwich formats; pair with a matched monoclonal antibody for improved specificity if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For GUCY2F-related studies in vision and cGMP signaling, we offer tailored sourcing of rare-target antibodies and custom development services to bridge your workflow from concept to clinic.