Anti-GUCY2D Polyclonal Antibody for WB and ELISA - Q02846

Core Product Specifications and Parameters

Biological Background: GUCY2D Function and Localization

• GUCY2D, also known as retinal guanylyl cyclase 1 (ROS-GC, GC-E), is a membrane-bound enzyme encoded by the GUCY2D gene in humans. Its multiple disease-associated aliases include LCA1, CORD6, and CSNB1I.
• GUCY2D catalyzes the conversion of GTP to cGMP, a key second messenger in photoreceptor cells, replenishing cGMP levels that are depleted during phototransduction in rods and cones. This activity is critical for visual signal transduction (PubMed:15123990, PubMed:21928830, PubMed:26100624). Related references: PMID:15123990 PMID:21928830 PMID:26100624
• Evidence suggests GUCY2D may also be involved in the trafficking of membrane-associated proteins to the photoreceptor outer segment membrane (By similarity).
• The protein localizes primarily to the photoreceptor outer segment membrane and the endoplasmic reticulum membrane, consistent with its role in sensory transduction and protein processing.
• GUCY2D is a glycoprotein containing multiple transmembrane helices, a disulfide bond, and a signal peptide, characteristic of its membrane integration and secretory pathway processing.
• Mutations in GUCY2D are associated with several inherited retinal dystrophies, including Leber congenital amaurosis (LCA1), cone-rod dystrophy (CORD6), and congenital stationary night blindness, underscoring its essential role in vision.

Experimental Guidance and Technical Tips

• Immunogen design: The recombinant fusion protein immunogen corresponds to the N-terminal region (aa 52-350) of human GUCY2D, which may target the extracellular/lumenal domain. This rational design ensures antibody specificity while avoiding highly hydrophobic transmembrane regions.
• Western blot (WB): A band around 120 kDa is expected based on the calculated molecular weight of ~120 kDa. Due to glycosylation and other modifications, actual migration may vary. Use proper controls and validate in retinal or photoreceptor-derived samples.
• ELISA: The antibody is suitable for ELISA. Consider indirect ELISA format and validate using recombinant protein or cell lysates expressing GUCY2D. Cross-reactivity with mouse and rat orthologs can be advantageous for translational studies.
• Sample preparation: For membrane proteins like GUCY2D, ensure adequate solubilization with appropriate detergents and avoid boiling samples to prevent aggregation. Optimize denaturing conditions for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates your IVD assay development with reliable, one-stop access to critical raw materials including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our GUCY2D Rabbit pAb supports research in vision science and retinal disease, and we excel in sourcing rare-target reagents to meet your unique diagnostic needs. Partner with CamelBio to streamline your supply chain from concept to clinic.