Anti-GUCA2B Polyclonal Antibody for WB, ELISA - Q16661

Core Product Specifications and Parameters

Biological Background: GUCA2B Function and Localization

• GUCA2B, also designated uroguanylin (UGN) and guanylyl cyclase activator 2B (GCAP-II), is a secreted protein hormone of approximately 12 kDa that belongs to the guanylin family.
• It functions as an endogenous activator of the intestinal guanylyl cyclase C receptor (GUCY2C), sharing the binding site with bacterial heat-stable enterotoxins.
• Upon receptor activation, GUCA2B stimulates intracellular cGMP production, regulating fluid and electrolyte transport across intestinal epithelia.
• GUCA2B is expressed predominantly in the stomach and intestine, where it acts in an autocrine/paracrine manner to modulate salt and water homeostasis.
• Structurally, GUCA2B contains disulfide bonds essential for its biologically active conformation, as indicated by its presence in 3D-structure and direct sequencing records.
• The mature peptide is derived from a larger precursor that includes a signal sequence, and it is detected as a secreted factor in biological fluids.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human GUCA2B, which includes the signal peptide region. This may allow recognition of both precursor and mature forms; consider validating band patterns in relevant tissue lysates (e.g., stomach or intestinal mucosa) using positive and negative controls.
• For Western blotting, run samples under reducing conditions and expect major bands compatible with the mature ~12 kDa peptide, possibly accompanied by precursor forms. Because of the small size, ensure adequate transfer and blotting conditions.
• The antibody cross-reacts with mouse GUCA2B, supporting its use in comparative studies. However, always verify specificity in the actual experimental system.
• In ELISA applications, the antibody can be tested as a capture or detection reagent; paired antibody formats may require additional optimization for sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering comprehensive support for diagnostic manufacturers, laboratories, and research institutes. For investigators focusing on gastrointestinal hormone signaling and intestinal fluid regulation, we provide a reliable supply of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, streamlining your path from concept to clinical application.