Anti-GUCA2A Polyclonal Antibody for WB and ELISA - Q02747

Core Product Specifications and Parameters

Biological Background: Guanylin Function and Localization

• Guanylin, also known as guanylate cyclase activator 2A (GUCA2A) or guanylate cyclase-activating protein I (GCAP-I), is a small secreted peptide hormone encoded by the GUCA2A gene in humans.
• It serves as an endogenous activator of intestinal guanylate cyclase, playing a central role in the regulation of fluid and electrolyte homeostasis within the gastrointestinal tract.
• Guanylin binds to the same receptor region as bacterial heat-stable enterotoxins, stimulating cyclic GMP production and triggering downstream signaling cascades.
• The protein is highly expressed in the ileum and colon and is also detectable in plasma, reflecting its local and systemic endocrine functions.
• The mature peptide contains characteristic disulfide bonds that are essential for maintaining its bioactive conformation.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against a recombinant protein spanning amino acids 22–115 of human GUCA2A, covering the proguanylin region and the mature peptide. It is expected to detect both precursor and mature forms in relevant assays.
• For Western blotting under reducing conditions, a band near 12 kDa may be observed; however, post-translational processing could yield smaller fragments. Additional optimization may be required for optimal detection.
• In ELISA applications, this antibody can be employed as a capture or detection reagent. We recommend validating the assay in the relevant sample matrix (e.g., intestinal tissue lysates, plasma) and using appropriate controls.
• Cross-reactivity with mouse and rat guanylin has been demonstrated, enabling utility in rodent models for gastrointestinal research.
• As with any polyclonal antibody, performance may vary between lots; include positive and negative controls in each experiment to ensure consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a reliable partner for guanylin-targeted studies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your gastrointestinal and enterotoxin research programs. Let CamelBio be your bridge to high-quality, supply-chain-ready IVD solutions.