Anti-Guanylyl Cyclase beta 1 (GUCY1B3) Rabbit Polyclonal Antibody for WB and ELISA - Q02153

Core Product Specifications and Parameters

Biological Background: Guanylyl Cyclase beta 1 (GUCY1B3) Function and Localization

• Guanylyl Cyclase beta 1 (GUCY1B3), also known as guanylate cyclase soluble subunit beta-1, soluble guanylate cyclase small subunit, or GCS-beta-3, is encoded by the GUCY1B1 gene (also designated GUC1B3, GUCSB3, GUCY1B3) in humans.
• The enzyme functions as the beta subunit of the heterodimeric soluble guanylate cyclase (sGC) complex, which serves as the primary receptor for nitric oxide (NO) and catalyzes the conversion of GTP to the second messenger cyclic GMP (cGMP).
• cGMP biosynthesis mediated by sGC plays a critical role in various physiological processes, including vasodilation, neurotransmission, and platelet aggregation.
• The protein localizes to the cytoplasm, where it is poised to respond to NO signals generated by NO synthases or exogenous sources.
• At the protein level, GUCY1B3 is detected in the brain cortex and cerebellum, indicating a role in neural signaling.
• The UniProt record for Q02153 notes alternative splicing events, direct protein sequencing evidence, and a resolved 3D structure, supporting its well-characterized biochemical properties.
• Key structural features include a heme-binding domain and metal-binding sites, which are essential for NO sensing and cGMP production.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1-250), which includes the regulatory heme-binding domain. This design may favor detection of both full-length and potential truncated isoforms.
• For Western blot, consider using a validated secondary antibody and appropriate loading controls. The predicted molecular weight is 71 kDa (as per product data), but post-translational modifications or dimerization may affect migration.
• For ELISA, confirm reactivity with the target protein in the specific sample matrix. Due to cross-reactivity with mouse and rat, this antibody may be suitable for comparative studies across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with trusted access to IVD raw materials, streamlining the journey from concept to clinical application. This high-quality anti-GUCY1B3 polyclonal antibody supports nitric oxide signaling and cGMP pathway research, and we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare target sourcing to meet your developmental needs. Partner with us to efficiently secure the critical reagents your projects demand.