Anti-Guanylyl Cyclase beta 1 (GUCY1B3) Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q02153

Core Product Specifications and Parameters

Biological Background: Guanylyl Cyclase beta 1 Function and Localization

• Guanylate cyclase soluble subunit beta-1 (GUCY1B3) is the protein product of the GUCY1B1 gene; it is also known as GCS-beta-3, soluble guanylate cyclase small subunit, and Guanylyl Cyclase beta 1.
• This subunit forms a heterodimer with an alpha subunit to constitute soluble guanylate cyclase, the primary receptor for nitric oxide (NO). Upon NO binding, the enzyme catalyzes the conversion of GTP to cGMP, a key second messenger mediating vasodilation, neurotransmission, and other physiological processes.
• GUCY1B3 is a member of the adenylyl cyclase-4/guanylyl cyclase family and contains a heme-binding domain that senses NO, a central GTPase region, and a catalytic cyclase domain.
• At the subcellular level, the protein localizes predominantly to the cytoplasm, consistent with its role as a soluble enzyme.
• Tissue specificity data indicate expression in the brain, particularly in the cortex and cerebellum, where nitric oxide-cGMP signaling regulates synaptic plasticity and neuronal function.
• Although the UniProt record lists alternative splicing as a keyword, no specific isoforms are described for this subunit beyond the canonical sequence.
• Keywords associated with GUCY1B3 include 3D-structure, cGMP biosynthesis, cytoplasm, heme, iron, metal-binding, and GTP-binding, reflecting its enzymatic and regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen consists of a synthetic peptide spanning the N-terminal region (amino acids 1–100), suggesting detection of full-length GUCY1B3; verify specificity with appropriate controls.
• For Western blotting, target band is expected at approximately 71 kDa (canonical molecular weight ~70.5 kDa); consider using reducing and non-reducing conditions to assess potential dimerization artifacts.
• In IHC-P, optimize antigen retrieval protocols and validate staining patterns in positive tissues such as brain cortex and cerebellum, which express GUCY1B3 at detectable levels.
• IF/ICC experiments should yield a cytoplasmic signal; co-localization studies with organelle markers may help confirm subcellular distribution.
• ELISA applications may utilize the antibody as a capture or detection reagent; pair with an anti-alpha subunit or anti-GUCY1B3 antibody from a different host for sandwich assays.
• Given cross-reactivity with mouse and rat, this antibody is suitable for studies in common rodent models; however, validate performance in each species’ tissue or cell line due to potential sequence variations.
• As soluble guanylate cyclase activity depends on heme and NO, consider whether your experimental conditions (e.g., cell stimulation with NO donors) might affect epitope accessibility or protein conformation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials. This anti-GUCY1B3 monoclonal antibody is one of thousands of validated, application-tested products we supply for nitric oxide signaling and cardiovascular research. Beyond individual antibodies, our one-stop platform offers curated antibody pairs, bulk ancillary reagents, and custom sourcing for rare or difficult-to-obtain targets, ensuring you can move from concept to clinic with a single, reliable partner.