Anti-GTPBP8 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8N3Z3

Core Product Specifications and Parameters

Biological Background: GTPBP8 Function and Localization

• GTPBP8 (GTP-binding protein 8) is a member of the GTP-binding protein family, encoded by the GTPBP8 gene.
• It binds guanosine triphosphate (GTP) and requires magnesium as a cofactor for its activity, as indicated by the keywords GTP-binding, Magnesium, and Metal-binding.
• Alternative splicing generates multiple isoforms of GTPBP8, expanding its functional diversity.
• The protein has a molecular weight of approximately 32 kDa (calculated from sequence), consistent with the observed 32kDa band in experiments.
• GTPBP8 has been identified in high-throughput proteomics studies (Proteomics identification), confirming its expression in human cells.
• Its specific biological role remains under investigation, but GTP-binding proteins generally participate in signal transduction, protein synthesis, and cellular regulation.
• As part of the reference proteome, GTPBP8 is a representative protein of the human proteome.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fusion protein spanning amino acids 1-170; it may recognize multiple epitopes and is suitable for Western blot (WB), immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), and ELISA.
• For WB, consider loading 20-30 µg of protein lysate and expect a band at approximately 32 kDa; validate with a positive control (e.g., HeLa or HEK293 cell lysate).
• For IHC-P and IF/ICC, optimize antigen retrieval (heat-induced, pH 6 or 9) and permeabilization (Triton X-100 or methanol) based on the sample type; include no-primary and tissue-specific controls.
• In ELISA applications, pre-coat with the recombinant immunogen or tissue/cell lysates, and verify reactivity against GST-tagged or full-length GTPBP8 to confirm specificity.
• Cross-reactivity with human, mouse, and rat is expected; always validate in the relevant species and tissue context before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing high-quality IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-GTPBP8 rabbit polyclonal antibody is part of our expanding portfolio for GTPase signaling research and diagnostic development. Trust CamelBio to streamline your supply chain from concept to clinic.