Anti-GTPBP4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9BZE4

Core Product Specifications and Parameters

Biological Background: GTPBP4 Function and Localization

• GTPBP4, also known as Nucleolar GTP-binding protein 1 (NOG1), Chronic renal failure gene protein (CRFG), and GTP-binding protein NGB, is a member of the GTP-binding protein family.
• Functions as a GTPase in the biogenesis of the 60S ribosomal subunit, essential for ribosome assembly and translation (PubMed:32669547). Related references: PMID:32669547
• Acts as a repressor of tumor protein TP53, preventing its stabilization and thereby inhibiting cell cycle arrest (PubMed:20308539). Related references: PMID:20308539
• Predominantly localizes to the nucleolus, the site of ribosome biogenesis.
• Post-translational modifications include acetylation, phosphorylation, and isopeptide bond formation (ubiquitin-like conjugation), which may regulate its activity and stability.
• The protein contains GTP-binding and nucleotide-binding domains, classifying it as a nucleolar GTPase.
• Alternative splicing of the GTPBP4 gene yields multiple isoforms, though specific isoform functions are not fully characterized.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 385-634 of human GTPBP4, covering the C-terminal region. This region shares homology with mouse and rat, likely contributing to the observed cross-reactivity.
• For Western blot (WB), the predicted molecular weight is 74 kDa; use appropriate gel percentage and loading controls. Validate the antibody with a positive control lysate.
• In immunohistochemistry (IHC-P), consider optimizing antigen retrieval methods (e.g., heat-induced epitope retrieval) and include a no-primary control to assess background staining.
• For immunofluorescence (IF/ICC), since GTPBP4 is nucleolar, permeabilization with detergents like Triton X-100 is recommended to access the nuclear compartment. Confirm localization with a nuclear marker.
• ELISA and other immunoassays may require antibody titration and blocking buffers optimized for polyclonal antibodies to reduce non-specific binding.
• Cross-reactivity with mouse and rat should be confirmed in the relevant tissue or cell line with species-specific positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for IVD raw materials, streamlining the sourcing of validated antibodies like this GTPBP4 polyclonal antibody for ribosome biogenesis and cancer research. Beyond antibodies, we supply optimized monoclonal/polyclonal reagent pairs, bulk ancillary materials, and can source rare-target raw materials to support your diagnostic and research workflows from concept to clinic.