Anti-GTPBP4 Monoclonal Antibody for WB, IHC-P, ELISA - Q9BZE4

Core Product Specifications and Parameters

Biological Background: GTPBP4 Function and Localization

• GTPBP4 (GTP-binding protein 4), also known as NOG1, NGB, and CRFG, is a nucleolar GTPase belonging to the Obg/CgtA GTPase subfamily. The gene generates isoforms through alternative splicing.
• It plays a critical role in the biogenesis of the 60S large ribosomal subunit. Related references: PMID:32669547
• GTPBP4 functions as a negative regulator of TP53 by preventing its stabilization, thereby inhibiting TP53-mediated cell cycle arrest. Related references: PMID:20308539
• The protein localizes predominantly to the nucleolus within the nucleus, consistent with its role in ribosome assembly.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (Ubl conjugation), as identified by proteomics approaches.
• Keywords from UniProt highlight its involvement in GTP-binding, nucleotide-binding, ribosome biogenesis, and nuclear localization, along with evidence at the protein level (direct protein sequencing).

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant fragment covering amino acids 2-92 of human GTPBP4, located near the N-terminus. This region may be suitable for detecting multiple isoforms, but users should verify isoform specificity in their experimental system.
• For Western blot (WB), the predicted molecular weight is 74 kDa. Consider using appropriate positive controls (e.g., human cell lines with known GTPBP4 expression) and validate the band pattern.
• In immunohistochemistry (IHC-P), expect nuclear/nucleolar staining based on the protein's subcellular localization. Optimization of antigen retrieval and antibody dilution may be required for specific tissue types.
• For ELISA assays, the recombinant immunogen can serve as a positive control to establish standard curves.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with integrated access to IVD raw materials for ribosomal biology and cancer research. For GTPBP4 detection, we supply validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents to support assay development. Our sourcing network also covers rare targets and bulk custom preparations to meet your project needs.