Anti-GTPBP10 Rabbit Polyclonal Antibody for WB and ELISA - A4D1E9

Core Product Specifications and Parameters

Biological Background: GTPBP10 Function and Localization

• GTPBP10 (GTP-binding protein 10), also known as ObgH2 or UG0751c10, is a member of the Obg family of GTPases that participate in ribosome biogenesis.
• It contributes to ribosome maturation, as shown by its ability to complement ObgE(CgtA) function in E. coli ribosome assembly.
• In vitro, GTPBP10 exhibits GTPase activity, suggesting it acts as a molecular switch in cellular processes.
• The protein localizes predominantly to the nucleus and nucleolus, and also associates with chromosomes, aligning with its role in ribosomal subunit production.
• Disruption of GTPBP10 function leads to disorganization of nucleolar architecture, emphasizing its structural importance in the nucleolus.
• The gene undergoes alternative splicing, and the protein features conserved GTP-binding domains typical of the Obg family.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is 43 kDa; however, actual migration may differ due to post-translational modifications or splice variants. Validate band specificity with appropriate controls.
• In ELISA applications, consider optimizing coating conditions and antibody titration to achieve the desired signal-to-noise ratio.
• The immunogen (aa 1-387) covers the N-terminal region; ensure that the target protein in your experimental system contains this epitope region.
• As a polyclonal antibody, batch-to-batch variability may occur; verify performance with your specific sample type and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research laboratories, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For targets like GTPBP10, involved in ribosome biology and nucleolar organization, we offer consistent supply and can assist with sourcing rare or custom raw materials to support your assay development from concept to clinic.