Anti-GTPBP1 Rabbit Polyclonal Antibody for WB, ELISA - O00178

Core Product Specifications and Parameters

Biological Background: GTPBP1 Function and Localization

• GTP-binding protein 1 (GTPBP1) is a GTPase that participates in the elongation phase of protein synthesis. It forms ternary complexes with GTP and aminoacyl-transfer RNAs (aa-tRNAs) and delivers aa-tRNAs to the ribosomal A site in a GTP-dependent manner.
  Related references:
  PMID:30108131

• GTPBP1 can also deliver deacylated tRNA to the A site. It is involved in RNA quality control by promoting exosomal degradation of faulty mRNAs engaged in 80S elongation complexes after GTP hydrolysis.
  Related references:
  PMID:30108131

• Additionally, GTPBP1 plays a role in the regulation of circadian mRNA stability (by similarity).

• The protein localizes to the cytoplasm, consistent with its function in translation.

• GTPBP1 contains GTP-binding and nucleotide-binding domains and exhibits RNA-binding activity, as indicated by UniProt keywords.

• Post‑translational modifications include phosphorylation; the protein has been identified in proteomics studies.

• GTPBP1 is associated with intellectual disability, as annotated in the UniProt record.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein spanning amino acids 10–280, covering the N‑terminal region of human GTPBP1. The resulting polyclonal antibody is expected to recognize multiple epitopes on the target protein.
• For Western blotting (WB) with a predicted molecular weight of 72 kDa, consider using whole‑cell lysates from human or mouse cell lines, given the reported cross‑reactivity.
• In ELISA applications, optimization of coating antigen and detection conditions is recommended to achieve the desired sensitivity and specificity.
• As with any polyclonal antibody, include appropriate negative controls and validate performance in the specific sample matrix and experimental system.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with reliable access to high‑quality IVD raw materials. This GTPBP1 polyclonal antibody addresses the growing interest in translation regulation and RNA quality control targets. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and undertake custom sourcing for rare targets—providing a streamlined procurement experience from concept to clinic.