Anti-GTF3C6 Polyclonal Antibody for WB, ELISA - Q969F1

Core Product Specifications and Parameters

Biological Background: GTF3C6 Function and Localization

• General transcription factor 3C polypeptide 6 (GTF3C6) is also known as Transcription factor IIIC 35 kDa subunit (TFIIIC35) and TFIIIC subunit 6, encoded by the GTF3C6 gene (aliases: C6orf51, bA397G5.3).
• It is a tightly associated component of the DNA-binding TFIIIC2 subcomplex, which is essential for RNA polymerase III-mediated transcription of tRNA and virus-associated RNA genes.
• The protein directly binds to tRNA and virus-associated RNA promoters as part of the TFIIIC complex.
• GTF3C6 is localized exclusively in the nucleus, consistent with its role in transcription.
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics data.
• The protein has a molecular weight of approximately 24 kDa (UniProt: 24049 Da, supplier: 24kDa).
• Keywords associated with this protein include DNA-binding, transcription, and nucleus, highlighting its function in gene regulation.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider using whole cell lysates from human or mouse cell lines expressing GTF3C6. Optimize primary antibody dilution and detection conditions.
• For ELISA, the immunogen design (N-terminal region, amino acids 1-213) suggests the antibody may recognize native protein; validate with recombinant protein or appropriate cell lysates.
• Given cross-reactivity with mouse, this antibody is suitable for comparative studies across species; always include appropriate controls.
• Confirm antibody specificity by pre-adsorption with the immunogen or by using siRNA knockdown/CRISPR knockout cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing platform for IVD raw materials, including this anti-GTF3C6 polyclonal antibody for research in RNA polymerase III transcription and TFIIIC complex biology. From validated antibodies to bulk ancillary reagents and rare target materials, we streamline procurement to support your complete workflow—from early development through clinical application.