Anti-GTF3C4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UKN8

Core Product Specifications and Parameters

Biological Background: GTF3C4 Function and Localization

• GTF3C4 (also designated TFIIIC90, KAT12, TF3C-delta) is a component of the general transcription factor IIIC (TFIIIC) complex, which is essential for RNA polymerase III (Pol III)-mediated transcription.
• It plays a critical role in the synthesis of small nuclear and cytoplasmic RNAs, including 5S rRNA, tRNA, and adenovirus-associated VA RNA. Related references: PMID:10523658
• GTF3C4 possesses intrinsic histone acetyltransferase (HAT) activity with unique specificity for free and nucleosomal histone H3, contributing to chromatin remodeling during transcription. Related references: PMID:10523658
• The protein cooperates with GTF3C5 to facilitate the recruitment of TFIIIB and RNA polymerase III through direct interactions with BRF1, POLR3C, and POLR3F. Related references: PMID:10523658
• Subcellular localization is predominantly nuclear, consistent with its role in transcription.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, as identified by proteomics and direct protein sequencing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 583–822 of human GTF3C4. For Western blot, the predicted molecular weight is approximately 92 kDa; however, actual migration may vary depending on gel conditions and post-translational modifications. Include appropriate positive and negative controls.
• For immunofluorescence (IF)/ICC, optimize permeabilization and fixation protocols for your specific cell line to ensure nuclear staining.
• The antibody shows cross-reactivity with rat, making it suitable for rodent model studies; validate in the intended sample system.
• As a polyclonal antibody, lot-to-lot consistency should be verified, and dilution factors should be determined empirically for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for diagnostic applications. For transcription-focused projects, our portfolio includes reliable tools like the GTF3C4 Rabbit pAb, enabling efficient development from concept to clinic.