Anti-GTF3C3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC - Q9Y5Q9

Core Product Specifications and Parameters

Biological Background: GTF3C3 Function and Localization

• GTF3C3 (General transcription factor 3C polypeptide 3) is a ~101 kDa subunit of the RNA polymerase III transcription factor complex, also known as TFIIIC102, TFIIICγ, or TFiiiC2-102.
• It is an integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex, essential for promoter recognition and transcription initiation by RNA polymerase III.
• The protein directly binds to tRNA gene promoters and virus-associated RNA promoters, enabling assembly of the preinitiation complex at these intragenic control regions.
• GTF3C3 is localized to the nucleus, consistent with its role in transcription.
• The protein contains TPR repeat motifs, is subject to acetylation and phosphorylation, and has been linked to intellectual disability when mutated (UniProt keywords: TPR repeat, Acetylation, Phosphoprotein, Disease variant, Intellectual disability).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 160 amino acids of human GTF3C3; this region may provide specificity for the target in WB applications after denaturing electrophoresis.
• For IHC-P, consider testing various antigen retrieval methods and validating staining in a known positive tissue, as nuclear antigens can be sensitive to fixation and processing.
• When performing IF/ICC, include appropriate nuclear counterstaining and confirm expected nuclear localization in the cell line or tissue under study.
• In ELISA setups, optimize coating antigen concentrations and antibody dilutions to achieve the desired sensitivity and avoid non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with dependable sourcing of IVD raw materials—from validated antibody pairs and optimized mono-/polyclonal antibodies to bulk ancillary reagents. For targets like GTF3C3 involved in transcription regulation and rare disease research, CamelBio streamlines procurement and technical consultation, helping you move from concept to clinic efficiently.