Anti-GTF3A Polyclonal Antibody for WB, ELISA - Q92664

Core Product Specifications and Parameters

Biological Background: GTF3A Function and Localization

• GTF3A (Transcription Factor IIIA) is a zinc-finger protein essential for RNA polymerase III-dependent transcription of 5S ribosomal RNA genes; it binds the internal control region (ICR) of the 5S rRNA gene. Related references: PMID:24120868
• It also associates with the transcribed 5S rRNA, suggesting a role in rRNA processing or ribosome assembly.
• Encoded by the GTF3A gene, it contains multiple C2H2-type zinc-finger motifs that mediate both DNA and RNA binding.
• Localizes predominantly to the nucleus, consistent with its function in transcriptional regulation.
• Ubiquitously expressed in human tissues, underpinning its fundamental role in ribosome biogenesis.
• Post-translational modifications and alternative splicing may produce variant isoforms; the canonical form migrates at approximately 42 kDa.
• Classified under keywords: alternative splicing, DNA-binding, metal-binding, nucleus, ribosome biogenesis, RNA-binding, transcription, zinc-finger.

Experimental Guidance and Technical Tips

• The immunogen spans the N‑terminal region (aa 1–100), so epitope specificity is predicted; consider confirming via peptide competition or genetic validation in your assay system.
• For Western blot, expect a band around 42 kDa; optimize gel percentage and transfer conditions for low‑molecular‑weight proteins.
• In ELISA, use standard indirect detection; titrate the antibody to determine the optimal working concentration.
• Cross‑reactivity is reported for human; for other species, experimental validation is recommended due to sequence divergence in the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For transcription and ribosome biogenesis research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities extend to rare target raw materials, ensuring your procurement needs are met efficiently.