Anti-GTF2IRD1 Rabbit Polyclonal Antibody for WB, ELISA - Q9UHL9

Core Product Specifications and Parameters

Biological Background: GTF2IRD1 Function and Localization

• GTF2IRD1 (General transcription factor II-I repeat domain-containing protein 1), also known as MusTRD1/BEN or WBSCR11, is a member of the TFII-I family of transcription factors characterized by multiple helix-loop-helix repeat domains.
• It functions as a transcriptional regulator implicated in cell-cycle progression and skeletal muscle differentiation, potentially repressing GTF2I by altering its nuclear localization or activation potential.
• In myogenesis, GTF2IRD1 contributes to slow-twitch fiber type specification and is active in regenerating muscle, binding the USE B1 enhancer of the troponin I slow gene.
• The protein also binds with high affinity to EFG sequences from the HOXC8 early enhancer, suggesting a broader role in developmental gene regulation.
• GTF2IRD1 is predominantly localized in the nucleus, consistent with its role as a DNA-binding transcription regulator.
• Tissue expression is highest in adult skeletal muscle, heart, fibroblasts, bone, and fetal tissues, with lower levels in other tissues.
• Post-translational modifications include phosphorylation and ubiquitination, and the protein undergoes alternative splicing to produce multiple isoforms.
• Its association with Williams-Beuren syndrome highlights its importance in neurodevelopmental and craniofacial processes.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the C-terminal region (aa 660-890) of human GTF2IRD1; the antibody may recognize epitopes conserved across isoforms and in rat protein.
• For Western blot, use lysates from tissues with high GTF2IRD1 expression (e.g., skeletal muscle, heart) and expect bands around 104–107 kDa.
• ELISA can be used for titer determination or recombinant protein detection, but coating and detection conditions should be optimized empirically.
• As a polyclonal antibody, lot-to-lot variability may occur; validate in your specific experimental system with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized polyclonal antibodies like this anti-GTF2IRD1, and bulk ancillary reagents. Whether you need rare target sourcing or technical consulting from concept to clinic, CamelBio streamlines your supply chain for transcription factor and developmental biology research.