Anti-GTF2IRD1 Polyclonal Antibody for WB, ELISA - Q9UHL9

Core Product Specifications and Parameters

Biological Background: GTF2IRD1 Function and Localization

• GTF2IRD1 (General transcription factor II-I repeat domain-containing protein 1) is a transcription regulator containing multiple repeat domains, encoded by the GTF2IRD1 gene located in the Williams-Beuren syndrome critical region.
• It plays a role in cell-cycle progression and skeletal muscle differentiation, potentially repressing GTF2I transcriptional functions by preventing its nuclear localization or inhibiting its activation.
• The protein contributes to slow-twitch fiber type specificity during myogenesis and in regenerating muscles, binding the troponin I slow-muscle fiber enhancer (USE B1).
• GTF2IRD1 binds with high affinity to EFG sequences in the early enhancer of HOXC8 (By similarity).
• It localizes to the nucleus.
• Highly expressed in adult skeletal muscle, heart, fibroblast, bone, and fetal tissues, with lower expression in other tissues.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond), and the gene undergoes alternative splicing.
• Associated with Williams-Beuren syndrome (WBS).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 660–959) of human GTF2IRD1, providing target specificity; consider verifying reactivity with relevant isoforms or truncation mutants if needed.
• For Western blot, expect a band around 106 kDa; include appropriate positive controls from tissues with high GTF2IRD1 expression, such as skeletal muscle or heart, and use a prestained protein ladder for accurate sizing.
• For ELISA, coat with the recombinant immunogen or target protein and validate detection across the species indicated (human, mouse, rat); consider checkerboard titrations to optimize antibody and antigen concentrations.
• The polyclonal nature may yield robust signal but lot-to-lot variation; we recommend including a reference antibody and consistently preparing samples under reducing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials, supporting projects from concept to clinic. This anti-GTF2IRD1 polyclonal antibody serves as a valuable tool for studying transcriptional regulation in muscle development and Williams-Beuren syndrome. Explore our portfolio for bulk antibody orders, validated pairs, and ancillary reagents to streamline your assay development.