Anti-GTF2I Polyclonal Antibody for WB, IF/ICC, ELISA - P78347

Core Product Specifications and Parameters

Biological Background: GTF2I Function and Localization

• GTF2I (General transcription factor II-I) is a multifunctional transcription regulator, also known as BAP-135, SPIN, and TFII-I, encoded by the GTF2I gene. Multiple isoforms exist due to alternative splicing, with isoform 1 showing strong expression in fetal brain.
• Acts as a signal-dependent coactivator by coordinating multiprotein complexes at the C-FOS promoter, linking specific activator complexes to serum-inducible transcription via the serum response element (SRE).
• Functions as a coregulator for USF1 by independently binding to a pyrimidine-rich initiator (Inr) and an upstream E-box, modulating transcription of target genes.
• Essential for B-lymphocyte activation: required for the formation of functional ARID3A DNA-binding complexes and activation of immunoglobulin heavy-chain transcription.
• Subcellular localization is both cytoplasmic and nuclear, reflecting its role in signal transduction and transcriptional regulation.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, influencing its activity and interactions.
• Associated with Williams-Beuren syndrome (WBS), a developmental disorder caused by a deletion at chromosome 7q11.23 that includes the GTF2I gene.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 600-810 of human GTF2I, a region likely conserved among species, supporting cross-reactivity with mouse and rat.
• For Western blot (WB), consider preliminary antibody dilution optimization; the predicted molecular weight is approximately 112 kDa.
• Immunofluorescence/immunocytochemistry (IF/ICC) may require optimization of fixation and permeabilization protocols due to both nuclear and cytoplasmic localization of GTF2I.
• ELISA can be utilized for quantitative detection; validate assay conditions in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-GTF2I polyclonal antibody supports research into transcription regulation, immunoglobulin gene control, and Williams-Beuren syndrome. Partner with CamelBio to streamline your IVD development from concept to clinic.