Anti-GTF2H4 Rabbit Polyclonal Antibody for WB, ELISA - Q92759

Core Product Specifications and Parameters

Biological Background: GTF2H4 Function and Localization

• GTF2H4 encodes the 52 kDa subunit of the general transcription factor IIH (TFIIH) core complex, also known as BTF2 p52 or TFIIH basal transcription factor complex p52 subunit.
• The TFIIH complex plays a dual role in transcription initiation by RNA polymerase II and in nucleotide excision repair (NER) of DNA damage. In transcription, it facilitates promoter opening and promoter escape, while in NER, it opens DNA around lesions to enable repair.
• GTF2H4 specifically stimulates the ATPase activity of the TFIIH subunit XPB/ERCC3, enhancing the complex's helicase function critical for both transcription and repair. Related references: PMID:17466626
• The protein localizes to the nucleus, consistent with its role in DNA transactions.
• Mutations in TFIIH components, including potentially GTF2H4, are associated with Xeroderma pigmentosum, a disorder linked to defective DNA repair.
• Alternative splicing results in multiple transcript variants, as suggested by the "Alternative splicing" keyword, though specific isoforms are not fully characterized.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein encompassing amino acids 1-300 of human GTF2H4. For Western blot, the predicted molecular weight is 52 kDa; validate the band in relevant nuclear extracts from human or mouse cells.
• ELISA applications may require optimization for coating antigen and detection conditions; consider testing with purified recombinant protein or cell lysates.
• Given the cross-reactivity with mouse, this antibody can be utilized for comparative studies between human and mouse samples, but always verify performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and researchers. For targets like GTF2H4 involved in transcription and DNA repair pathways, we support rare target raw-material sourcing and offer technical consulting to accelerate development from concept to clinic.