Anti-GTF2H3 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q13889

Core Product Specifications and Parameters

Biological Background: GTF2H3 Function and Localization

• GTF2H3 encodes the 34kDa subunit of the general transcription and DNA repair factor IIH (TFIIH) core complex, also known as Basic transcription factor 2 34kDa subunit (BTF2 p34), TFIIH basal transcription factor complex p34 subunit, and P34.
• As part of TFIIH, GTF2H3 plays a dual role: in transcription initiation, it is essential for promoter opening and promoter escape by RNA polymerase II; in nucleotide excision repair (NER), it contributes to DNA unwinding around lesions, enabling damaged oligonucleotide excision and repair synthesis.
• TFIIH consists of a core complex and a kinase module (CAK); GTF2H3 is a core component critical for the structural integrity and function of the entire TFIIH assembly.
• The protein localizes to the nucleus, consistent with its functions in transcription and DNA damage response.
• GTF2H3 contains a zinc-finger domain, suggesting metal-binding activity, and undergoes alternative splicing, potentially generating functionally distinct isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–308) of human GTF2H3. Antibodies raised against this region may recognize full-length protein as well as N-terminally conserved splice variants.
• For Western blotting, consider enriching nuclear extracts to improve detection sensitivity, given the nuclear localization of GTF2H3.
• In immunoprecipitation, nuclear lysis buffers optimized for transcription factor complexes may preserve TFIIH interactions; validate antibody performance with target-specific knockdown or knockout controls.
• For ELISA applications, the antibody can be used either for antigen capture or detection with appropriate recombinant GTF2H3 protein or nuclear extracts; optimization of coating and blocking conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for high-quality IVD raw materials, offering validated antibody pairs, recombinant proteins, and ancillary reagents to support assay development and biomarker studies. For transcription and DNA repair research, we supply this anti-GTF2H3 polyclonal antibody along with related antibodies, proteins, and technical services, streamlining your transition from concept to clinic.