Anti-GTF2H1 Polyclonal Antibody for WB, IF/ICC, ELISA - P32780

Core Product Specifications and Parameters

Biological Background: GTF2H1 Function and Localization

• GTF2H1, also known as p62 or BTF2, is the 62 kDa subunit of the general transcription factor IIH (TFIIH) core complex, which plays dual roles in RNA polymerase II-mediated transcription and nucleotide excision repair (NER).
• In transcription initiation, TFIIH is required for promoter opening and escape; the associated CAK kinase module phosphorylates the C-terminal domain (CTD) of RNA polymerase II, enabling the transition to elongation.
• In DNA repair, TFIIH unwinds DNA around damage sites to facilitate lesion excision, functioning in both global genome and transcription-coupled NER pathways.
• The protein localizes predominantly to the nucleus, consistent with its involvement in transcription and DNA repair processes.
• Post-translational modifications, including acetylation, phosphorylation, and ubiquitin conjugation, likely modulate GTF2H1 stability, activity, and interactions within the TFIIH complex.
• Alternative splicing of the GTF2H1 gene yields multiple isoforms, which may contribute to functional diversity in different cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–425, encompassing a large portion of the full-length protein (62 kDa). For Western blot, anticipate a band near 62 kDa; inclusion of untreated and DNA-damaged cell lysates may help confirm specificity.
• Given its polyclonal nature, signal intensity may vary between lots; consider validating new lots with known positive and negative controls.
• For immunofluorescence/immunocytochemistry, nuclear staining is expected; optimize fixation and permeabilization conditions to preserve antigenicity.
• The antibody shows cross-reactivity with mouse and rat, making it suitable for studies in these model organisms; however, validation in the specific experimental system is advised.
• ELISA applications may require preliminary checkerboard titrations to determine optimal coating antigen and antibody concentrations.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive, one-stop supply chain for IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. This anti-GTF2H1 polyclonal antibody exemplifies our commitment to offering high-quality reagents for transcription and DNA repair research.
• Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal formulations, bulk ancillary reagents, and rare target raw-material sourcing. Partner with CamelBio to streamline your IVD development and accelerate time to market.