Anti-GTF2F2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P13984

Core Product Specifications and Parameters

Biological Background: GTF2F2 Function and Localization

• GTF2F2 encodes the beta subunit of the general transcription factor IIF (TFIIF), also known as TFIIF-beta or RAP30.
• TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps recruit it to the pre-initiation complex in collaboration with TFIIB.
• The TFIIF complex consists of two subunits: the large alpha subunit (GTF2F1) and the small beta subunit (GTF2F2), which together regulate transcription initiation and elongation.
• GTF2F2 localizes to the nucleus, consistent with its role in transcription.
• Post-translational modifications, including acetylation and phosphorylation, have been identified, suggesting regulatory control of TFIIF activity.
• The protein possesses DNA-binding ability, likely contributing to promoter recognition and stabilization of the transcription complex.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human GTF2F2. Consider verifying the antibody's specificity using peptide blocking assays or knockdown controls.
• In Western blotting, the antibody detects a band at approximately 28 kDa, matching the predicted molecular weight. Validate in relevant cell lines expressing GTF2F2.
• For immunohistochemistry (IHC-P), perform antigen retrieval and expect nuclear staining. Use appropriate positive (e.g., human tonsil) and negative controls.
• For ELISA, optimize the antibody concentration and coating conditions using recombinant GTF2F2 protein as standard.
• Cross-reactivity with mouse and rat is reported; confirm reactivity in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated monoclonal antibodies like this anti-GTF2F2 antibody, along with technical services and consulting to advance your transcription research projects. From concept to clinic, CamelBio streamlines procurement of bulk reagents and rare targets, ensuring reliable supply chains for your diagnostic development needs.