Anti-GTF2F2 Polyclonal Antibody for WB, IF/ICC, ELISA - P13984

Core Product Specifications and Parameters

Biological Background: GTF2F2 Function and Localization

• GTF2F2 encodes the beta subunit of general transcription factor IIF (TFIIF-beta), also referred to as RAP30 or BTF4.
• TFIIF-beta heterodimerizes with the alpha subunit (GTF2F1) to form intact TFIIF, which directly binds RNA polymerase II.
• TFIIF facilitates the recruitment of RNA polymerase II to promoter preinitiation complexes by cooperating with TFIIB, thereby regulating accurate transcription initiation.
• The protein is predominantly localized in the nucleus, consistent with its role in transcription.
• Post-translational modifications including acetylation and phosphorylation have been reported, potentially modulating TFIIF activity or interactions.
• As a DNA-binding protein, TFIIF-beta may contribute to promoter recognition and start-site selection during early transcription.
• It belongs to the TFIIF-beta family and is widely studied as a component of the basal transcription machinery.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human GTF2F2 sequence (aa 1-249), raising polyclonal antibodies that recognize multiple epitopes along the protein.
• For western blot (WB), GTF2F2 migrates at approximately 28 kDa; ensure proper size markers and loading controls are used.
• In immunofluorescence/immunocytochemistry (IF/ICC), nuclear staining is expected; optimize fixation and permeabilization conditions for your cell type.
• For ELISA, recombinant GTF2F2 protein can serve as a positive control to assess antibody binding and establish standard curves.
• Reported cross-reactivity is for human; if applying to other species, verify sequence homology and validate experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a single-point supply of IVD raw materials, including this high-quality anti-GTF2F2 rabbit polyclonal antibody. Our catalog features validated antibody pairs, optimized mono- and polyclonal antibodies, and bulk ancillary reagents, alongside custom sourcing capabilities for rare transcription factor targets. Partner with us to streamline your assay development from concept to clinic.