Anti-GTF2E2 Polyclonal Antibody for WB, ELISA - P29084

Core Product Specifications and Parameters

Biological Background: GTF2E2 Function and Localization

• GTF2E2, also known as TFIIE-beta, is a subunit of the general transcription factor IIE (TFIIE) complex essential for RNA polymerase II-mediated transcription initiation.
• It recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain (CTD) kinase and DNA-dependent ATPase activities of TFIIH.
• Together with TFIIH, it is required for promoter clearance, enabling RNA polymerase II to transition from initiation to elongation.
• Encoded by the GTF2E2 gene (GeneID: 2961) in humans, the protein has a predicted molecular weight of approximately 33 kDa and localizes to the nucleus.
• Post-translational modifications include acetylation and phosphorylation, and it is known to bind DNA directly.
• Mutations in GTF2E2 are associated with trichothiodystrophy (TTD6), a disease variant noted in UniProt.
• Conserved across human, mouse, and rat, making it a useful target for cross-species transcription studies.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human GTF2E2 protein (aa 1-291), so the resulting polyclonal antibody may recognize multiple epitopes; consider this advantage for detecting potential isoforms or degradation products.
• For Western blot, expect a band at approximately 33 kDa; validate specificity using knockout or knockdown controls in human, mouse, or rat cell lysates.
• For ELISA, the antibody can be employed as either capture or detection reagent; optimize conditions for your specific assay format and antigen concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti-GTF2E2 polyclonal antibody alongside a wide range of transcription-related reagents. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your procurement. Our one-stop service supports your journey from concept to clinic, ensuring reliable access to high-quality materials for transcription research and diagnostic development.