Anti-GTF2E1 Polyclonal Antibody for WB, IF, ELISA - P29083

Core Product Specifications and Parameters

Biological Background: GTF2E1 Function and Localization

• GTF2E1 encodes the alpha subunit of transcription factor IIE (TFIIE-alpha), a 439-amino acid protein with a molecular mass of approximately 49 kDa (also known as the 56 kDa subunit).
• As part of the TFIIE complex, it recruits TFIIH to the RNA polymerase II pre-initiation complex and stimulates TFIIH's C-terminal domain kinase and DNA-dependent ATPase activities, essential for promoter clearance.
• TFIIE-alpha contains zinc-finger motifs and is a metal-binding phosphoprotein, with reported acetylation and phosphorylation modifications that may regulate its activity in transcription.
• The protein localizes to the nucleus, consistent with its central role in transcription initiation.
• GTF2E1 is involved in host-virus interactions (according to UniProt keywords), indicating a potential role in viral transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 230–439), which may result in antibodies that recognize specific isoforms or domains; verify specificity in the relevant sample system.
• For Western blot (WB), an expected band near 49 kDa should be detected in human, mouse, and rat lysates; consider using appropriate positive and negative controls.
• For immunofluorescence/ICC, anticipate nuclear staining; optimize fixation and permeabilization conditions for the cell type of interest.
• In ELISA applications, the recombinant immunogen can serve as a positive control; titer optimization may be required.
• Cross-reactivity with mouse and rat suggests potential for multi-species studies, but it is recommended to validate in each species' relevant tissue or cell line.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-GTF2E1 polyclonal antibody is designed for transcription research and diagnostic assay development. Through CamelBio's one-stop platform, diagnostic manufacturers and labs can efficiently access rare target raw materials and technical support to accelerate their projects from concept to clinic.