Anti-GTF2B Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q00403

Core Product Specifications and Parameters

Biological Background: GTF2B Function and Localization

• GTF2B (General transcription factor IIB) is a universally required transcription factor that plays a central role in the initiation of RNA polymerase II (Pol II) transcription. It is essential for the formation of the pre-initiation complex (PIC) and recruitment of Pol II to promoter DNA. Related references: PMID:12931194 PMID:1517211 PMID:1876184
• Together with TATA-box binding protein (TBP), GTF2B forms the core initiation complex and bridges TBP to the Pol II-TFIIF complex, enabling accurate transcription start site selection. Related references: PMID:8413225 PMID:8504927 PMID:8515820 PMID:10318856
• GTF2B binds directly to specific DNA core promoter elements, known as the upstream (BREu) and downstream (BREd) IIB-recognition elements, independent of TBP, which helps position the transcription machinery. Related references: PMID:10619841 PMID:16230532 PMID:7675079
• After transcription initiation, GTF2B is released early from the PIC during the transition to elongation and reassociates with TBP to prepare for the next round of transcription. Related references: PMID:7601352
• GTF2B possesses autoacetyltransferase activity, which contributes to the activation of transcription. Related references: PMID:12931194
• The protein localizes to the nucleus and associates with chromatin at core promoter-specific regions. It is expressed in the inner cell mass of the embryoblast but not in outer trophoblast cells, indicating a role in early development. Related references: PMID:12931194 PMID:24441171
• GTF2B contains a zinc-finger domain and exhibits a repetitive structure, classified under keywords such as metal-binding, DNA-binding, transcription, and host-virus interaction.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant protein corresponding to amino acids 40–154 of human GTF2B. This region may be conserved across species, supporting reactivity with mouse and rat as indicated.
• For Western blotting, a band around 35 kDa is expected. Consider using appropriate positive control lysates (e.g., HeLa, HEK293) where GTF2B is expressed.
• In immunohistochemistry (IHC) and immunofluorescence (IF/ICC), nuclear staining should be observed. Validate in the relevant sample system to confirm specificity.
• For ELISA applications, optimize coating antigen and antibody concentrations based on the target protein context.
• Always perform preliminary experiments to determine optimal dilutions and conditions for your specific assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, supplying manufacturers and labs with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials. This anti-GTF2B antibody supports transcription research and diagnostic assay development, complementing our extensive catalog of molecular biology reagents. Contact us to streamline your procurement from concept to clinic.