Anti-GTF2A2 Rabbit Polyclonal Antibody for WB and ELISA - P52657

Core Product Specifications and Parameters

Biological Background: GTF2A2 Function and Localization

• Encodes the gamma subunit (also called p12 subunit) of general transcription factor IIA (TFIIA), essential for RNA polymerase II-dependent transcription initiation.
• TFIIA forms a complex with the TATA-box binding protein (TBP) and mediates transcriptional activation.
• Localized exclusively in the nucleus, consistent with its role in transcription regulation.
• Listed under keywords: transcription, transcription regulation, and reference proteome, underscoring its fundamental role in gene expression.
• Has a resolved 3D structure, providing insights into its molecular interactions within the pre-initiation complex.
• Implicated in host-virus interactions, highlighting its potential involvement in viral transcription or replication processes.
• Identified by proteomics approaches, confirming its expression in human cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-109) of human GTF2A2; this region may be suitable for detecting both full-length and any known isoforms.
• For Western blot, the predicted molecular weight is ~12 kDa; ensure appropriate gel percentage and transfer conditions for small proteins.
• ELISA validation suggests the antibody can recognize native or denatured target; consider optimizing coating conditions for sandwich assays.
• Given the nuclear localization, nuclear extracts or enriched fractions may improve detection sensitivity.
• Cross-reactivity is confirmed for human; if using in other species, verify sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material sourcing, offering a comprehensive portfolio from validated antibodies to ancillary reagents. This GTF2A2 rabbit polyclonal antibody supports transcription machinery studies and assay development. Our supply chain solutions cover everything from bulk monoclonals to hard-to-find target raw materials, accelerating your diagnostic projects from concept to clinic.