Anti-GTF2A1 Rabbit Polyclonal Antibody for WB and ELISA - P52655

Core Product Specifications and Parameters

Biological Background: GTF2A1 Function and Localization

• GTF2A1 encodes the 42 kDa subunit 1 of transcription initiation factor IIA (TFIIA), also known as TFIIA-42 or TFIIAL.
• TFIIA is a nuclear protein complex that acts as a component of the RNA polymerase II transcription machinery, playing a critical role in transcriptional activation.
• In conjunction with the TATA-box binding protein (TBP), TFIIA mediates the formation and activity of the transcription pre-initiation complex.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, which may modulate its activity.
• Alternative initiation of translation can produce different isoforms of GTF2A1.
• Subcellular localization is exclusively nuclear, consistent with its role in DNA-templated transcription.
• Keywords: 3D-structure, Acetylation, Alternative initiation, Direct protein sequencing, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Transcription, Transcription regulation.

Experimental Guidance and Technical Tips

• This antibody was generated using a recombinant protein spanning amino acids 1-270 of GTF2A1 (NP_056943.1), which covers the majority of the protein's N-terminal region. Consider performing peptide blocking or epitope mapping if fine specificity is required.
• For Western blot applications, a band of approximately 42 kDa is expected in human cell or tissue lysates. Validation in the relevant sample system and inclusion of appropriate controls is recommended.
• ELISA applications may utilize the antibody as a detection reagent; however, optimal coating conditions and detection limits should be empirically determined.
• Cross-reactivity is reported for human samples; caution is advised when applying to other species without prior validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers and research institutes as a comprehensive provider of IVD raw materials, offering a curated selection of validated antibodies, bulk reagents, and technical support. For researchers focused on transcription initiation and gene regulation, CamelBio can source GTF2A1 antibodies and ancillary reagents to streamline assay development. As your sourcing partner, CamelBio helps bridge the gap from concept to clinical application, ensuring reliable supply of rare and established targets.