Anti-GSTZ1 Rabbit Polyclonal Antibody for WB, ELISA - O43708

Core Product Specifications and Parameters

Biological Background: GSTZ1 Function and Localization

• GSTZ1, also known as maleylacetoacetate isomerase or glutathione S-transferase zeta 1, is a member of the glutathione S-transferase (GST) superfamily, specifically the zeta class.
• It functions as a bifunctional enzyme with both maleylacetoacetate isomerase activity, critical in the phenylalanine/tyrosine catabolic pathway, and glutathione S-transferase activity towards substrates like ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole.
• Additionally, it possesses low glutathione peroxidase activity with organic hydroperoxides and can catalyze the glutathione-dependent oxygenation of dichloroacetic acid to glyoxylic acid, a reaction relevant to the metabolism of environmental contaminants.
• GSTZ1 is predominantly localized in the cytoplasm.
• Tissue distribution is broad but highest in liver, kidney, skeletal muscle, and brain; also found in melanocytes, synovium, placenta, breast, and fetal liver and heart.
• According to UniProt, GSTZ1 undergoes post-translational modifications including acetylation and phosphorylation, and has alternative splicing isoforms.
• Keywords: Isomerase, Transferase, Multifunctional enzyme, Phenylalanine catabolism, Tyrosine catabolism, Acetylation, Alternative splicing, Cytoplasm, Disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to nearly full-length GSTZ1 (aa 1-216); sequence conservation across human, mouse, and rat may support cross-reactivity, but validation in the target species is recommended.
• For WB, the predicted molecular weight is 24 kDa; use an appropriate gel percentage and include positive controls such as human liver or cell lysates known to express GSTZ1.
• For ELISA, consider using recombinant GSTZ1 protein as a positive control, and optimize coating conditions and antibody dilutions empirically.
• Since GSTZ1 is a cytoplasmic enzyme, prepare protein extracts accordingly and consider subcellular fractionation to enrich the signal.

CamelBio: Your One-Stop Sourcing Bridge

• As a dedicated IVD raw-material supplier, CamelBio provides validated GSTZ1 antibodies and can support the development of diagnostic assays targeting metabolic and detoxification pathways.
• From polyclonal antibodies to recombinant protein reagents and ancillary materials, we offer a streamlined procurement pipeline for research and diagnostic applications.
• Leverage our expertise to source rare-target antibodies and optimize reagent performance for your specific project needs.